Extensive understanding of ion channel responses in serial applications of agonists and/or antagonist is dependent upon advanced abilities in solution control and precise fluidic manipulation. These capabilities have traditionally resided in the domain of slow but reliable single cell patch clamp techniques due to technologically limiting factors plaguing most automated patch clamp (APC) systems. Even with the implementation of microfluidics, precise timing in fluidic exchange while consistently applying continuous solution flow has been elusive to most high throughput APC systems, limiting the scope of most drug research in that domain.
By eliminating the need for external liquid handlers for in-assay solution exchange, IonFlux systems have always utilized a unique in-plate continuous perfusion and precise parallel application of compounds while clamping and recording from ensembles of cells in whole-cell or single cell patch clamp formation. Here we present case studies showing among others the effect of continuous flow on high throughput drug binding research in voltage gated channels and the importance of precision in fluidic exchange on stability of highly desensitizing ligand gated receptors.