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Importance of fluidic control and precision in serial activation of ionic channels
Extensive understanding of ion channel responses in serial applications of agonists and/or antagonist is dependent upon advanced abilities in solution control and precise fluidic manipulation. These capabilities have traditionally resided in the domain of slow but reliable single cell patch clamp techniques due to technologically limiting factors plaguing most automated patch clamp (APC) systems. Even with the implementation of microfluidics, precise timing in fluidic exchange while consistently applying continuous solution flow has been elusive to most high throughput APC systems, limiting the scope of most drug research in that domain.
By eliminating the need for external liquid handlers for in-assay solution exchange, IonFlux systems have always utilized a unique in-plate continuous perfusion and precise parallel application of compounds while clamping and recording from ensembles of cells in whole-cell or single cell patch clamp formation. Here we present case studies showing among others the effect of continuous flow on high throughput drug binding research in voltage gated channels and the importance of precision in fluidic exchange on stability of highly desensitizing ligand gated receptors.

Aug 22, 2019 11:00 AM in Eastern Time (US and Canada)

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Speakers

Ali Yehia, Ph.D.
VP IonFlux @Fluxion Biosciences
Ali is an experienced electrophysiologist with an extensive background in automated patch clamp. He has been working in the industry for more than 13 years. Before joining Fluxion Biosciences, he worked at Sophion Bioscience for more than 5 years, first as an Application Scientist and Scientific Business Manager and ending as the Director of Sales for North America. Ali received his PhD in Physiology (Cardiac Electrophysiology) from McGill University in Montreal, Canada and is now the VP for IonFlux at Fluxion Biosciences.